Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTP/MPP<sup>+</sup>-Induced Toxicity via Progesterone Receptor Signaling

<div>Immunofluorescence analysis of PR expression. (a) Detection of PR expression. The model of MPTP-induced parkinsonism was treated with puerarin (120 mg/kg/day), progesterone (8 mg/kg/day), or RU486 (3 mg/kg/day), as indicated. Midbrain cryosections were probed with primary anti-PR antibody and visualized with Alexa Fluor 594-conjugated secondary antibody, whereas the cell nuclei were stained with DAPI. The substantia nigra areas were examined under a Zeiss LSM 780 confocal microscopy. Representative images with 20x amplification were shown. Scale bar, 100 <i>μ</i>m. (b) Quantitative analysis of PR levels. 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Oxidative Medicine and Cellular Longevity

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Figure 3

Figure 3: Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTP/MPP<sup>+</sup>-Induced Toxicity via Progesterone Receptor Signaling 

Figure 3 | Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTP/MPP+-Induced Toxicity via Progesterone Receptor Signaling 