Botanical Drug Puerarin Promotes Neuronal Survival and Neurite Outgrowth against MPTP/MPP⁺-Induced Toxicity via Progesterone Receptor Signaling

Figure 5

Puerarin promoted neurite outgrowth in primary midbrain neurons. (a) Visualization of neurite outgrowth. Primary midbrain neurons were treated with puearin and RU486 as indicated. Following immunofluorescence staining with anti-GAP43 antibody and DAPI, the cells were examined under a fluorescence microscope from Carl Zeiss (Jena, Germany). Scale bar, 50 μm. (b) Quantification of neurite outgrowth. The neurite length was quantified by using ImageJ (http://imageJ.nih.gov). Neurites from three nonoverlapping fields for each slice were recorded and analyzed for (a). .

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