Triterpenoid Saponin AG8 from Ardisia gigantifolia stapf. Induces Triple Negative Breast Cancer Cells Apoptosis through Oxidative Stress Pathway

Figure 3

Effects of AG8 on mitochondrial function in TNBC cells. (a) MDA-MB-231, BT-549, and MDA-MB-157 cells were incubated with 0.1% DMSO or with AG8 for 24 h. Following treatment, cells were incubated with 100 nM MitoTracker Red CMXRos for 30 min. The cells were observed by fluorescence microscope (100x). (b) Intracellular ROS generation induced by AG8 and NAC was measured by DCFH-DA (10 μM) and flow cytometry. All results are representative of three independent experiments, , , and versus control.

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