High-Fat Diet Aggravates Acute Pancreatitis via TLR4-Mediated Necroptosis and Inflammation in Rats

Figure 1

HFD aggravated the histological injury and oxidative stress in the pancreas of acute pancreatitis rats. (a) AP was induced in SCD or HFD rats with a standard retrograde infusion of STC into the biliopancreatic duct; the animals were killed for analysis 3 h, 6 h, and 12 h after the STC infusion. (b) HFD rats showed a larger volume of adipocytes in the peripancreatic fat than SCD rats after an 8-week feeding. (c) In the AP group, serious edema (blue arrow), massive acinar necrosis, inflammatory cell infiltration (black arrowhead), and intrapancreatic hemorrhage (yellow arrow) in the pancreas were observed. (d) Pathological score of pancreatic tissues in rats. (e) Percentage of necrotic cells was determined in the pancreas. (f) Serum levels of free fatty acid in rats. (g) Pancreatic levels of MDA. (h) Pancreatic levels of LPO. (i) Pancreatic activities of SOD. (j) Pancreatic levels of GSH. versus prior subgroup, ^# versus SCD group at the same time point. Scale .

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