High-Fat Diet Aggravates Acute Pancreatitis via TLR4-Mediated Necroptosis and Inflammation in Rats

Figure 5

Inhibition of TLR4 attenuated inflammatory response and necroptosis in the pancreas of HFD rats with AP. (a) TLR4 and RIP3 levels of pancreas were analyzed by Western blot. β-Actin served as the loading control. (b) The relative protein expression of TLR4 was expressed as fold changes compared with the control in the blot after normalized to β-actin. (c) The relative protein expression of RIP3 was expressed as fold changes compared with the control in the blot after normalized to β-actin. (d) Immunostaining of NF-κB p65, TNF-α, MPO, and CD68 in the pancreas of HFD rats. (e) The expression of NF-κB p65-positive nuclei in the pancreas. (f) The mean IOD of TNF-α in the pancreas. (g) Number of MPO-positive neutrophils. (h) Number of CD68-positive macrophages. versus HV group, ^# versus HAP group. Scale .

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