Amelioration of Cigarette Smoke-Induced Mucus Hypersecretion and Viscosity by Dendrobium officinale Polysaccharides In Vitro and In Vivo

Figure 4

Effect of DOPs on CS-induced mucus secretion and viscosity in in vitro model. Primary normal human bronchial epithelial cells (HBECs) after 28-day air-liquid interface (ALI) culture were treated with DOPs or N-acetylcysteine (NAC) and then 4% cigarette smoke medium (CSM) for 24 hours. (a) Analysis of MUC5AC mRNA expression was carried out by real-time qPCR. (b) MUC5AC protein was quantitated by ELISA in apical washing. (c) Mucus viscosity was measured using a Brookfield LVDV-II+Pro cone and plate digital viscometer with CP-40 spindle in apical washing. Values are expressed as (). , , and for one-way ANOVA test with post hoc analysis (Bonferroni). ^#, ^##, and ^### for Student’s -test.

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