Differential Gender-Dependent Patterns of Cardiac Fibrosis and Fibroblast Phenotypes in Aging Mice

Figure 2

Gender-dependent patterns of cardiac fibrosis with age. (a): Perivascular coronary, epicardial and myocardial histological sections stained in Masson’s trichrome obtained from young and aged mice of both sexes, as well as quantifications of interstitial reactive, replacement and epicardial fibrosis, and coronary adventitial thickness. The green stains represent the fibrotic areas. (b): Representative images and quantifications of immunofluorescence staining (594 nm) for whecat germ agglutinin (WGA) in the hearts from young and aged mice of both sexes. Nuclei are stained blue with DAPI. Scale bars: 100 μm in (a) and 50 μm in (b). Magnifications: x100 in (a) and x200 in (b). animals per group, fields of view per condition. and vs young male and female, , and vs aged female or male.

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