Investigation of Chemical Composition, Antioxidant Activity, and the Effects of Alfalfa Flavonoids on Growth Performance

Table 1

Identification of flavonoid compounds in 75% EEFs from alfalfa.

No.	RT (min)	[M-H]^- ()	Exact mass	Formula	Major product ions ()	Proposed compounds	Compared with standard, literature, MassBank
1	22.10	609	610	C27H30O16	300	Rutin	The three of them
2	22.67	463	464	C21H20O12	300; 271; 255	Hyperoside	The three of them
3	25.37	447	448	C21H20O11	284; 255; 227	Astragalin	The three of them
4	26.57	269	270	C15H10O5	133; 135	5,3,4-Trihydroxyflavone	The MassBank
5	28.81	461	462	C22H22O11	283; 285; 255	Chrysoeriol-7-O-glucoside	The literature, MassBank
6	32.81	255	256	C15H12O4	135; 119; 91	Liquiritigenin	The literature
7	34.58	253	254	C15H10O4	135; 117; 91	7,4-Dihydroxyflavone	The MassBank, standard
8	59.11	269	270	C15H10O5	117; 151	Apigenin	The literature, standard
9	62.29	299	300	C16H12O6	284; 256	Chrysoeriol	The literature, MassBank
10	63.02	329	330	C17H14O7	314; 299; 271	Tricin	The literature, MassBank
11	63.56	345	346	C17H14O8	330; 315	Limocitrin	The literature, MassBank
12	64.78	297	298	C17H14O5	282; 253	3,7-Dimethoxy-3-hydroxyflavone	The literature, MassBank
13	66.82	267	268	C16H12O4	252; 223; 195	Formononetin	The literature, MassBank
14	67.04	255	256	C15H12O4	135; 119; 91	Isoliquiritigenin	The literature, MassBank
15	73.80	299	300	C17H16O5	284; 271; 256	7,4-Dimethoxy-5-hydroxyflavanone	The MassBank