Protective Effect of miR-204 on Doxorubicin-Induced Cardiomyocyte Injury via HMGB1

Figure 6

miR-204 targets HMGB1 in DOX-induced cardiac injury. (a) The binding site of miR-204 and HMGB1. (b) The luciferase activity of HMGB1-UTR (HMGB1-WT) or mutant (HMGB1-MUT) in the indicated groups (), versus miR-NC. (c) Western blot analysis was performed to measure HMGB1 protein levels in hearts with or without miR-204 (), versus NS+GFP and ^# versus DOX+GFP. (d) Western blots and quantitative analysis of HMGB1 in each group (), versus PBS+miR-NC and ^# versus DOX+miR-NC.

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