Methane-Rich Saline Alleviates CA/CPR Brain Injury by Inhibiting Oxidative Stress, Microglial Activation-Induced Inflammatory Responses, and ER Stress-Mediated Apoptosis

Figure 5

MRS alleviated the inflammatory response in the hippocampus after CA/CPR. The levels of (a) TNF-α, (b) IL-6, and (c) IL-10 in hippocampal tissues were measured 6 h after CA/CPR using commercial ELISA kits. (d) The expression levels of TNF-α, IL-6, and IL-10 in hippocampal tissue protein at 6 h after CA/CPR. (e) Relative band intensity of TNF-α, IL-6, and IL-10. (f) iNOS immunohistochemistry staining to detect hippocampal CA1 region inflammation response. (g) Quantification of iNOS relative intensity area (%). Scale bars indicated 25 μm. Data were reported as . The statistical significance was assessed by the one-way ANOVA test ( and vs. sham control group; and ^# vs. MRS control group; ^& and ^$ vs. CPR+NS group).

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