Research Article
Metabolomic Analysis of the Ameliorative Effect of Enhanced Proline Metabolism on Hypoxia-Induced Injury in Cardiomyocytes
Figure 3
Enhanced proline metabolism induced by overexpression of PRODH decreases reactive oxidative stress, whereas PRODH knockdown has the opposite effect. (a) Representative images and quantitative analysis of reactive oxidative stress as assessed by flow cytometric analysis with DCFH fluorescent staining (). (b) Representative images and quantitative analysis of superoxide production as measured by DHE fluorescence. Scale bar, 100 μm (). The data are presented as the values. versus the normoxia control group; # versus the Ad-NC or Ad-shNC negative control group.
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