Effect of Anticancer Quinones on Reactive Oxygen Production by Adult Rat Heart Myocytes

Table 1

Requirements for quinone-enhanced oxygen consumption in rat heart myocytes. Oxygen consumption was determined polarographically in a 3 ml reaction system at 37°C that contained 125 mM potassium phosphate buffer, pH 7.4, 140 mM NaCl, 10 mM glucose, and viable cardiac myocytes.

Reaction system	Oxygen consumption (nmol O2/min/ cells)
Control	^a
+KCN (5 mM)	1^b
+Rotenone (10 μM)	^b
Doxorubicin (400 μM)
+Rotenone (10 μM)	^c
Doxorubicin (400 μM)
+KCN (5 mM)	^c
Doxorubicin (400 μM)
+KCN (5 mM)
-Cells	ND^d

^a of 3 to 5 experiments. ^bSignificantly different from the control, at . ^cSignificantly different from samples containing mitochondrial inhibitors alone, at . ^dN.D. is not detectable.