Increased Expression of BIRC2, BIRC3, and BIRC5 from the IAP Family in Mesenchymal Stem Cells of the Umbilical Cord Wharton’s Jelly (WJSC) in Younger Women Giving Birth Naturally

Figure 3

Example dot-plot graphs showing cell morphology and cell distribution in terms of expression intensity of cIAP1 and cIAP2 proteins as well as CD105 and CD90 surface antigens in a sample analyzed on cell culture day 10. (a) Dependence of the size of analyzed cells on their shape and granularity: FSC, forward scatter/SSC, side scatter. (b) cIAP2 vs. SSC: R48 cIAP2+. (c) cIAP1 vs. SSC: R47 cIAP1+. (d) cIAP1 vs. cIAP2: R6 cIAP1-/cIAP2+; R7cIAP1+/cIAP2+; R8 cIAP1-/cIAP2-; and R9 cIAP1+/cIAP2-. (e) CD105 vs. cIAP2: R18 CD105-/cIAP2+; R19 CD105+/cIAP2+; R20 CD105-/cIAP2-; and R21CD105+/cIAP2-. (f) CD105 vs. cIAP1: R14 CD105-/cIAP1+; R15CD105+/cIAP1+; R16 CD105-/cIAP1-; and R17 CD105+/cIAP1-. (g) CD90 vs. cIAP1: R34 CD90-/cIAP1+; R35 CD90+/cIAP1+; R36 CD90-/cIAP1-; and R37 CD90+/cIAP1-. (h) CD90 vs. cIAP2: R26 CD90-/cIAP2+; R27 CD90+/cIAP2+; R28 CD90-/cIAP2-; and R29 CD90+/cIAP2-. The color chart indicates the expression of the CD34+, CD90+, and CD105+ antigens, highlighting the coexpression of the surface antigens and proteins tested. Analysis and graph made using Summit™ Software digital flow cytometer together with the MoFlo XDP Beckman Coulter cell sorter.

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