Colchicine Alleviates Cholesterol Crystal-Induced Endothelial Cell Pyroptosis through Activating AMPK/SIRT1 Pathway

Figure 1

Colchicine suppresses cholesterol crystal-induced endothelial cell pyroptosis. (a–c) Impacts of increasing cholesterol crystals and colchicine concentrations on the viability of HUVECs. Endothelial cells treated with increasing doses of (a) cholesterol crystals (0-2.0 mg/ml) and (b) colchicine (0-100 nM) and (c) cholesterol crystal (0.5 mg/ml) and different concentrations of colchicine (0-10 nM) for 24 h. The cell viability was detected using a CCK8 assay. (d) Pyroptotic cell death was measured with Hoechst 33342 (blue)/PI (red) double-fluorescent staining (). (e) The LDH release was evaluated with a cytotoxicity detection LDH kit. (f) Transmission electron microscopy (TEM) was used to observe the pyroptotic cell morphology (scale bar  μm). Data was expressed as the of three separate experiments. , , and vs. the control group. ^#, ^##, and ^### vs. the cholesterol crystal group.

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