Nitric Oxide Stimulates Acute Pancreatitis Pain via Activating the NF-κB Signaling Pathway and Inhibiting the Kappa Opioid Receptor

Figure 2

Acute pancreatitis increases the level of SP and CGRP while reducing KOR levels. (a–c) Immunofluorescence staining of DRG sections of mice with or without acute pancreatitis. SP, CGRP, and Oprk1 are stained red, and the nuclei are stained blue. (d) The mRNA expression of Oprk1, SP, and CGRP in DRGs of mice with or without acute pancreatitis. The experiment was repeated three times. The data were analyzed using Student’s -test and one-way ANOVA and are presented as the normalized expression relative to the control group. , /group. (e) Protein expression of Oprk1, SP, and CGRP in DRGs and pancreatic tissue of mice in the control group and acute pancreatitis group as detected by western blot assay. A: gray values were detected by ImageJ (National Institutes of Health), and the data were analyzed using Student’s -test and one-way ANOVA and are presented as the normalized expression relative to the control group. , /group. (f) SP and CGRP levels in the serum of mice in the control group and acute pancreatitis group as detected by the ELISA kit. The data were analyzed using Student’s -test and one-way ANOVA and are presented as the normalized expression relative to the control group. , /group.