Nitric Oxide Stimulates Acute Pancreatitis Pain via Activating the NF-κB Signaling Pathway and Inhibiting the Kappa Opioid Receptor

Figure 3

NO promotes the expression of pain factors in mice with acute pancreatitis. (a–c) Immunofluorescence staining of DRG sections of mice with acute pancreatitis treated with the NO donor and scavenger. SP, CGRP, and Oprk1 are stained red, the nuclei are stained blue. (d) The mRNA expression of Oprk1, SP, and CGRP in DRGs of mice with acute pancreatitis treated with the NO donor and scavenger. The experiment was repeated three times. The data were analyzed using Student’s -test and one-way ANOVA and are presented as the normalized expression relative to the control group. , /group. (e) Protein expression of Oprk1, SP, and CGRP in DRGs and pancreatic tissue of mice with acute pancreatitis treated with the NO donor and scavenger as detected by western blot assay. Gray values were detected by ImageJ (National Institutes of Health), and the data were analyzed using Student’s -test and one-way ANOVA and are presented as the normalized expression relative to the control group. . This part of the experiment was carried out in mice with acute pancreatitis.