Nitric Oxide Stimulates Acute Pancreatitis Pain via Activating the NF-κB Signaling Pathway and Inhibiting the Kappa Opioid Receptor

Figure 4

Molecular mechanism by which NO promotes pain. (a) In vitro administration of DRGs with the NO donor and scavenger and western blot assay for the expression of NF-κB pathway-associated proteins. (b) In vitro administration of DRGs with the NF-κB pathway inhibitor and western blot assay for the expression of iNOS and Oprk1. (c) In vitro administration of DRGs with the KOR agonist and western blot assay for the expression of NF-κB pathway-associated proteins. Gray values were detected by ImageJ (National Institutes of Health), and the data were analyzed using Student’s -test and one-way ANOVA and are presented as the normalized expression relative to the control group. .

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