Nitric Oxide Stimulates Acute Pancreatitis Pain via Activating the NF-κB Signaling Pathway and Inhibiting the Kappa Opioid Receptor

Figure 5

NO increases the expression of pain factors through NF-κB. (a) Protein expression of Oprk1, SP, and CGRP in DRGs treated with the NO donor, scavenger, and NF-κB pathway inhibitor alone or combined as detected by western blot assay. (b) The mRNA expression of Oprk1, SP, and CGRP in DRGs treated with the NO donor, scavenger, and NF-κB pathway inhibitor alone or combined. The experiment was repeated three times. The data were analyzed using Student’s -test and one-way ANOVA and are presented as the normalized expression relative to the control group. compared to the control group, ^# compared to the SNP group. (c) Protein expression of SP and CGRP in DRGs treated with the NO scavenger, NF-κB pathway inhibitor, and KOR agonist alone or combined as detected by western blot assay. (d) The mRNA expression of SP and CGRP in DRGs treated with the NO scavenger, NF-κB pathway inhibitor, and KOR agonist alone or combined. The experiment was repeated three times. The data were analyzed using Student’s -test and one-way ANOVA and are presented as the normalized expression relative to the control group. .

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