Nitric Oxide Stimulates Acute Pancreatitis Pain via Activating the NF-κB Signaling Pathway and Inhibiting the Kappa Opioid Receptor

Figure 6

Effects of NO, NF-κB, and the KOR on pain behaviors in mice. (a) The writhing response of mice under different intervention conditions. The writhing response of mice was recorded within 30 minutes, and the experiment was repeated three times. The data were analyzed using Student’s -test and one-way ANOVA. compared to the control group, ^# compared to the AP group, /group. (b) The electromyography of mice under different intervention conditions. String stimulation was given 10 times at 0.05 V, and the electromyography was collected to analyze the changes in electromyography amplitude. The experiment was repeated three times, calculating the difference in electromyography amplitude, and the data were analyzed using Student’s -test and one-way ANOVA. compared to the control group, ^# compared to the AP group, /group. (c) The von Frey filament test of mice under different intervention conditions. The upper abdomen was stimulated 10 times with filaments of 0.04 g, 0.16 g, and 0.6 g. The data are expressed as the total score of the response caused by 10 repeated challenges with each hair and were analyzed using Student’s -test and one-way ANOVA. compared to the control group, ^# compared to the AP group, /group.

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