Dexmedetomidine Postconditioning Alleviates Hypoxia/Reoxygenation Injury in Senescent Myocardial Cells by Regulating lncRNA H19 and m⁶A Modification

Figure 1

H₂O₂ induced senescence of the myocardial cell line H9c2. (a) After 4 h exposure of H9c2 cells with the final concentrations of 0 μM, 75 μM, 150 μM, 300 μM, or 600 μM H₂O₂, medium was replaced with normal DMEM for 24 h. Cells were then stained with SA-β-gal, and proportions of blue cells were calculated (, ; , , vs. 0 μM). (b) A CCK-8 assay was used to detect the viability of each treatment group (, ; , , vs. 0 μM). (c) Expression of senescence-associated proteins P21 and P53 in each group was detected by western blot (, ; , ^# vs. 0 μM).

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