HSP70 Inhibition Leads to the Activation of Proteasomal System under Mild Hyperthermia Conditions in Young and Senescent Fibroblasts

Figure 4

Effects of KNK437 treatment on the protein levels of HSP70 in young and senescent fibroblast cells. After 12 hours of incubation with KNK437, cells were treated with mild hyperthermia at 42°C for 1 h and cultured at 37°C for 3 h. Protein levels were measured by western blotting followed by densitometric analysis of protein bands and relative ratios were normalized to β-actin levels in young (a) and senescent (b) fibroblast cells. A: DMSO control, B: KNK437 control, C: DMSO+42°C for 1 h with recovery at 37°C for 3 h, and D: KNK437+42°C for 1 h with recovery at 37°C for 3 h. Data are expressed as . vs. A; ^& vs. C ().

(a)

(b)