HSP70 Inhibition Leads to the Activation of Proteasomal System under Mild Hyperthermia Conditions in Young and Senescent Fibroblasts

Figure 5

Effects of HSP70 inhibition on proteasome activity in young and senescent fibroblast cells. After 12 hours of incubation with KNK437, cells were treated with mild hyperthermia at 42°C for 1 h and cultured at 37°C for 3 h. Proteasomal activity was measured in young (a) and senescent (b) fibroblast cells. A: DMSO control, B: KNK437 control, C: DMSO+42°C for 1 h with recovery at 37°C for 3 h, and D: KNK437+42°C for 1 h with recovery at 37°C for 3 h. Data are expressed as . vs. A; ^& vs. C ().

(a)

(b)