HSP70 Inhibition Leads to the Activation of Proteasomal System under Mild Hyperthermia Conditions in Young and Senescent Fibroblasts

Figure 6

2D gel images of proteins displaying differential levels in young and senescent fibroblast cells following heat stress. Cells were treated with mild hyperthermia at 42°C for 1 h and used immediately for analysis or cultured at 37°C for 3 h. Cells were harvested and protein spots were determined by two-dimensional gel electrophoresis (a) followed by identification of protein bands via MALDI-TOF MS (b).

(a)

(b)