The Regulatory Effect of MicroRNA-101-3p on Disc Degeneration by the STC1/VEGF/MAPK Pathway

Figure 3

Identification of STC1 as a target of miR-101-3p. (a) Venn diagram showing miR-101-3p computationally predicted to target STC1 by different algorithms. (b) The potential target of miR-101-3p accessed by Cytoscape. (c) Schematic diagram showed that the putative binding site of miR-101-3p within the 3-UTR of STC1 mRNA demonstrated the high conservation of miR-101-3p between different species. (d) Luciferase activity of wild- or mutant-type STC1 3-UTR reporter plasmid after transfections in NP cells. (e) The relative STC1 expression level evaluated by qRT-PCR after transfections. (f) The STC1 protein expression accessed by western blots after transfections. Quantitative analysis is shown on the right. Values presented as . . NP: nucleus pulposus.

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