The Suppression of Pin1-Alleviated Oxidative Stress through the p38 MAPK Pathway in Ischemia- and Reperfusion-Induced Acute Kidney Injury

Figure 1

Pin1 expression was increased during renal I/R injury in rats. The model was established with 15, 30, and 45 min ischemia and 24 h reperfusion. (a, b) The Cr and BUN levels in serum were detected. (c) The Pin1 mRNA level was increased in RT-PCR. (d, e) The Pin1 protein level was increased in Western blot, and the quantification was performed. (f, g) The SOD activity and MDA content were detected after I/R injury in rats. (h, i) The pathological change was examined by H&E staining and its quantification (×400). (j) The renal ROS level was examined by DHE staining (×400) (). The values were presented as . vs. the sham group.

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