Research Article
The Suppression of Pin1-Alleviated Oxidative Stress through the p38 MAPK Pathway in Ischemia- and Reperfusion-Induced Acute Kidney Injury
Figure 5
The Pin1 inhibitor, Juglone, regulated oxidative stress caused by H/R injury in vitro. The model was established with 12 h hypoxia and 6 h reoxygenation. HK-2 cells were treated with the Pin1 inhibitor (0.1,1, and 10 μM) for 1 h and then experienced the H/R process. (a) The effect of different concentrations of juglone on the control group. (b–f) Western blot was used to detect the expression of Pin1, 4-HNE, COX2, and MPO and quantification was performed. (g–j) The SOD, MDA, ROS, and H2O2 levels were detected after the H/R process in vitro (). The values were presented as . vs. the control group and vs. the H/R group.
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