The Suppression of Pin1-Alleviated Oxidative Stress through the p38 MAPK Pathway in Ischemia- and Reperfusion-Induced Acute Kidney Injury

Figure 6

Pin1 silence decreased oxidative stress induced by H/R in vitro. The H/R model was established with 12 h hypoxia and 6 h reoxygenation. The cells were transfected with si-NC or two different si-Pin1 for 24 h and then experienced H/R. (a–e) Western blot was used to detect the expression of Pin1, 4-HNE, COX2, and MPO and quantification was performed. (f–i) The SOD, MDA, ROS, and H₂O₂ levels were detected after H/R in vitro (). The values were presented as . vs. the control group and vs. the si-NC group.

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