Research Article
Folic Acid Protects Melanocytes from Oxidative Stress via Activation of Nrf2 and Inhibition of HMGB1
Figure 3
FA potentiated the antioxidant response in H2O2-treated PIG1 cells. PIG1 cells were exposed to 800 μM H2O2 and FA at indicated concentrations for 48 h simultaneously. (a, b) Intracellular ROS level was determined by flow cytometry assay. Bar graphs represent the mean values of the fluorescence intensity of ROS level. (c) The activity of antioxidant enzyme SOD was examined via using a specialized kit. (d) Effects of FA on H2O2-induced expressions of Nrf2, p-Nrf2, SOD2, and HO-1 were determined via Western blot. β-Actin was detected as loading control. (e, f) The nuclear/cytoplasmic distribution of Nrf2 was detected via Western blot (e) and immunofluorescence (f), respectively. Lamin A/C and tubulin were detected as loading controls in western blot assay. Nrf2 was stained with Cy3 (red) and nuclei were counterstained with DAPI (blue) in fluorescence assay. . All data are presented as the across three independent experiments. , , ; ns: not significant.
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