Alkyl Hydroperoxide Reductase as a Determinant of Parasite Antiperoxide Response in Toxoplasma gondii

Figure 2

Localization of AHP1 in T. gondii. (a) Strategy for constructing HA-tagged fusion-expressing strain. (b) Western blot confirming successful integration of the HA tag. (c) IFA used for TgAHP1 localization observation. TgAHP1 (green) and TgGAP45 (red) in RH∆Ku80 determined the localization. Scale bar, 5 μm. DAPI: 4,6-diamidino-2-phenylindole, nuclear dye; TgGAP45: gliding associated protein 45; BF: bright field.

(a)

(b)

(c)