Alkyl Hydroperoxide Reductase as a Determinant of Parasite Antiperoxide Response in Toxoplasma gondii

Figure 3

Catalase activity of AHP1 protein in T. gondii. (a) Western blot identification of the protein expressed by pET-Ahp1 recombinant plasmid using His monoclonal antibody. (b) CAT activity of TgAHP1 recombinant protein measured at concentrations of 1 mg/ml, 3 mg/ml, 5 mg/ml, and 7 mg/ml. (c) Predicted three-dimensional structure of the TgAHP1 protein. The yellow dots represent cysteines. (d) Western blot identification of the protein expressed by the pET-Ahp1 mutant recombinant plasmids using His monoclonal antibody. (e) CAT activity of TgAHP1 mutant recombinant proteins. Values shown are from three independent experiments ().

(a)

(b)

(c)

(d)

(e)