Alkyl Hydroperoxide Reductase as a Determinant of Parasite Antiperoxide Response in Toxoplasma gondii

Figure 4

Higher sensitivity of AHP1 to tBOOH than to H₂O₂. (a) In vitro detection of parasite invasion. The invading parasites were pretreated with different concentrations of H₂O₂ and tBOOH for 2 h. (b) Difference analysis of invasion efficiency treated with different concentrations of H₂O₂ and tBOOH. (c) Intracellular replication assay to examine parasite proliferation in vitro. The invading parasites were pretreated with H₂O₂ and tBOOH for 2 h, and the experiment was then repeated three times independently (). (d) The RH∆Ku80 strain was stimulated with tBOOH and H₂O₂, and the TgAhp1 gene expression was detected at 2, 8, 14, 20, and 26 h. Values shown are from three independent experiments (), each with three replicates. ns, indicates nonsignificant; ; ; ; all by Student’s -test.

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