Alkyl Hydroperoxide Reductase as a Determinant of Parasite Antiperoxide Response in Toxoplasma gondii

Figure 5

Characterization of an Ahp1 deletion strain. (a) Schematic diagram of Ahp1 knockout in RH∆Ku80 by CRISPR/Cas9-mediated homologous gene replacement. (b) Western blot confirming successful integration of the GFP tag using mouse anti-GFP antibody. (c) Difference invasion efficiency of RH∆Ku80 and ∆Ahp1 strains. (d) Intracellular replication assay for examining RH∆Ku80 and ∆Ahp1 strains proliferation in vitro.

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