Alkyl Hydroperoxide Reductase as a Determinant of Parasite Antiperoxide Response in Toxoplasma gondii

Figure 6

Attenuating the oxidative stimulation effect of tBOOH on T. gondii tachyzoites relies on AHP1: (a) Difference invasion efficiency of RH∆Ku80 and ∆Ahp1 strains. The invading parasites were pretreated with 10^-4 M tBOOH for 2 h. (b) Intracellular replication assay to examine parasite proliferation in vitro. The invading parasites were pretreated with tBOOH for 2 h, and the experiment was then repeated three times independently (). (c) A 7-day plaque assay was performed on ∆Ahp1 and the RH∆Ku80 strains after stimulation with 10^-4 M tBOOH. (d, e) Statistics of the number of plaques and plaque area of RH∆Ku80 strain and ∆Ahp1 strain formed using ImageJ software. (f) Survival curve of mice infected with the designated strains. The RH∆Ku80 and ∆Ahp1 strains stimulated with 10^-4 M tBOOH were used to infect Balb/C mice by intraperitoneal injection (500 tachyzoites per mouse, mice per strain), and the survival conditions of mice were recorded daily.

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