Alkyl Hydroperoxide Reductase as a Determinant of Parasite Antiperoxide Response in Toxoplasma gondii

Figure 7

tBOOH induced an increase in ROS levels and apoptosis in Ahp1-deficient parasites. (a) tBOOH induced ROS in the RH∆Ku80 and ∆Ahp1 strains. (b) Statistical analysis showed that the ∆Ahp1 strain produced significantly higher ROS than the RH∆Ku80 strain before 15 min. (c) Two lines real-time images of parasite apoptosis were observed through a fluorescence microscope in different visual field. (d) Statistical calculation of the apoptosis rate of ∆Ahp1 and RH∆Ku80 strains under tBOOH stimulation. The numbers of apoptotic tachyzoites in each visual field were counted to obtain the apoptosis rate of the different treatment groups. (e) tBOOH significantly induced apoptosis in the ∆Ahp1 strain after 15 min (). The apoptosis rates were calculated: . DCF: dichlorodihydrofluorescein; PI: propidium iodide. Scale bar, 5 μm.

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