Methylation of CALCA and CALCB in Pancreatic Ductal Adenocarcinoma

Figure 3

Detection of CGRP methylation by methylation-specific PCR (MSP): (a) CpG island methylation of CALCA was found by MSP electrophoresis in pancreatic cancer tissue. A: carcinoma; P: paracancer; M: methylation (target fragment: 132 bp); U: nonmethylation (target fragment: 158 bp). (b) CpG island methylation of CALCB was found by MSP electrophoresis in pancreatic cancer tissue. A: carcinoma; P: paracancer; M: methylation (target fragment: 143 bp); U: nonmethylation (target fragment: 105 bp). There is only one band of M in complete methylation, two bands of M and U in partial methylation, and only one band of U in nonmethylation.

(a)

(b)