Methylation of CALCA and CALCB in Pancreatic Ductal Adenocarcinoma

Table 1

Primer sequences used to amplify CGRP.

	Target gene (promoter region)	CALCA (NC_000011.10 (14966668…14972361, complement))
MSP	Forward	5-TTTTAGGTTTTGGAAGTATGAGGGTGATG-3
	Reverse	5-TTCCCACCACTATAAATCA-3
	Annealing temperature	53°C
USP	Forward	5-GTTTTGGAAGTATGAGGGTGACG-3
	Reverse	5-TTCCCGCCGCTATAAATCG-3
	Annealing temperature	53°C
	Target gene (promoter region)	CALCB (NC_000011.10 (15073593…15078637))
MSP	Forward	5-TTTTTAGAAAAGATGGATAGGTCGA-3
	Reverse	5-ACCAACACTCACTAAAACAAATACG-3
	Annealing temperature	45°C
USP	Forward	5-TTTTTAGAAAAGATGGATAGGTTGA-3
	Reverse	5-CCAACACTCACTAAAACAAATACAC-3
	Annealing temperature	45°C