SIRT1 Attenuates Apoptosis of Nucleus Pulposus Cells by Targeting Interactions between LC3B and Fas under High-Magnitude Compression

<div>High-magnitude compression aggravated cell apoptosis and attenuated SIRT1 expression in rat NPCs. (a) The main units of the substance exchanger-based perfusion bioreactor (1: substance exchanger; 2: medium reservoir; 3: tissue culture chambers and pressure stress application system; 4: peristaltic pump; 5: pH, PO<sub>2</sub>, and CO<sub>2</sub> sensor). (b, c) The apoptosis rate and SIRT1, p-P65, and apoptosis-related protein (Bcl-2, Bax, and cleaved caspase 3) levels in NPCs exposed to different compressive deformations (0% and 20%) were examined by flow cytometry and western blotting, respectively. (d) The expression of SIRT1 in NPCs exposed to different compressive deformations (0% and 20%) was examined by immunofluorescence. <svg height="10.7395pt" id="M4" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 102C330 152 324 179 321 236C368 205 390 186 422 144C444 143 470 148 486 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411V388C99 379 102 374 102 310V103C102 38 93 32 27 26V0H238V26C189 32 180 38 180 103V338C210 363 250 390 289 390C351 390 377 348 377 275V103C377 37 368 32 306 26V0H520V26C465 32 456 38 456 101V296C456 314 455 326 453 338C491 369 529 390 565 390C628 390 653 345 653 274V107C653 36 642 32 583 26V0H797Z"></path></g></svg>.</span> NPCs: nucleus pulposus cells.</div>

Oxidative Medicine and Cellular Longevity

fig1

Figure 1

Figure 1: SIRT1 Attenuates Apoptosis of Nucleus Pulposus Cells by Targeting Interactions between LC3B and Fas under High-Magnitude Compression 