RSL3 Drives Ferroptosis through NF-κB Pathway Activation and GPX4 Depletion in Glioblastoma

Figure 4

NF-κB pathway inhibition, using BAY 11-7082 (12.5 μM), reduced RSL3-induced ferroptosis in glioblastoma cells. (a) CCK-8 assay examined U87 and U251 cell viability at different concentrations of RSL3 for 24 h. (b) Lipid peroxidation analysis for U87 and U251 cells treated with 0.25 μM and 0.5 μM RSL3 for 24 h, respectively. (c) Western blot analysis of IκBα, p-IκBα, and mature IL-1β expression in U87 and U251 cells treated with 0.25 μM and 0.5 μM RSL3 for 24 h, respectively (d) Western blot analysis of ATF4, xCT, GPX4, and HO-1 expression in U87 and U251 cells treated with 0.25 μM and 0.5 μM RSL3 for 24 h, respectively. Experiments were performed thrice, and data is presented as the . .

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