Mangiferin Inhibits PDGF-BB-Induced Proliferation and Migration of Rat Vascular Smooth Muscle Cells and Alleviates Neointimal Formation in Mice through the AMPK/Drp1 Axis

Figure 4

Mangiferin alleviated PDGF-BB-induced mitochondrial fission and increase in ROS levels. (a) RSMCs were stained with mitotracker probe after treatment with different concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. The average length of mitochondria was quantified using ImageJ software. (b) Intracellular ROS levels were detected using flow cytometry after RSMCs were treated with mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. (c–e) Western blot was used to measure the protein expression levels of AMPK, pAMPKThr172, Drp1, and pDrp1Ser616 after RSMCs were treated with various concentrations of mangiferin with or without 20 ng/ml PDGF-BB for 24 hours. All assays were conducted in triplicate and all data presented as . , compared with the control group. ^#, compared with the PDGF-BB group.

(a)

(b)

(c)

(d)

(e)