[Retracted] Osthole Attenuates Bleomycin-Induced Pulmonary Fibrosis by Modulating NADPH Oxidase 4-Derived Oxidative Stress in Mice

Figure 6

OST inhibits TGF-β1-induced ROS burst in lung fibroblasts by inhibiting NOX4. (a) Expression of NOX4 in MRC-5 cells treated with TGF-β1 (10 ng/mL) and OST (1-50 μM) for 24 h (). (b–d) MRC-5 cells were treated with TGF-β1 (10 ng/mL), OST (20 μM), control, or NOX4 overexpression adenovirus (10moi) for 24 h. Expression of NOX4 was determined with Western blot () and immunofluorescence staining (, scale  μm); level of ROS was measured with a DCFH-DA-based assay kit (, scale  μm). vs. control group; ### vs. TGF-β1 group; ††† vs. TGF-β1 + OST group.

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