[Retracted] Osthole Attenuates Bleomycin-Induced Pulmonary Fibrosis by Modulating NADPH Oxidase 4-Derived Oxidative Stress in Mice

Figure 7

OST acts the antifibrosis effects by inhibiting ROS triggered Smad3 phosphorylation. (a–c) MRC-5 cells were treated with TGF-β1 (10 ng/mL), OST (20 μM), control, or NOX4 overexpression adenovirus (10moi) for 24 h. Then, the following assays were finished: determining level of p-Smad3 and total Smad3 with Western blot (); measuring level of α-SMA with immunofluorescence staining (, scale  μm); determining expression of α-SMA, CTGF, collagen I, and collagen III with Western blot (). (d) Proliferation of lung fibroblasts treated with TGF-β1 (10 ng/mL), OST (20 μM), control, or NOX4 overexpression adenovirus (10moi) for 48 h (). vs. control group; ## vs. TGF-β1 group; ### vs. TGF-β1 group; ††† vs. TGF-β1 + OST group.

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