Research Article

A Protein from Dioscorea polystachya (Chinese Yam) Improves Hydrocortisone-Induced Testicular Dysfunction by Alleviating Leydig Cell Injury via Upregulation of the Nrf2 Pathway

Figure 2

DP1 ameliorated testicular dysfunction in HCT-treated rats. (a) Histomorphological changes in testicular tissues. (b, c) Apoptosis levels of testicular cells were detected by the TUNEL assay. (d, e) The ratio of Bcl-2/Bax in testicular tissues was detected by Western blotting. (f) Masson staining for collagen fibers in testicular tissues. (e, g) The protein expression levels of TGF-β1 and p-SMAD2/3 in testicular tissues were detected by Western blotting. (h) Comparison of the testosterone content in rats. Each bar represents the . The images were captured at a magnification of ×100 or ×200. ## and ### vs. the control group; and vs. the HCT group.
(a)
(b)
(c)
(d)
(e)
(f)
(g)
(h)