Research Article
A Protein from Dioscorea polystachya (Chinese Yam) Improves Hydrocortisone-Induced Testicular Dysfunction by Alleviating Leydig Cell Injury via Upregulation of the Nrf2 Pathway
Figure 5
DP1 promoted the upregulation of the Nrf2/HO-1 pathway. (a, b) The protein expression levels of Nrf2 and HO-1 in testicular tissues were detected by Western blotting. (c) Relative mRNA expression of HO-1, NQO1, and GCLC normalized to GAPDH in TM3 cells. (d, e) The protein expression levels of total Nrf2, nuclear Nrf2, and nuclear HO-1 in TM3 cells were detected by Western blotting. Each bar represents the . The images were captured at a magnification of ×100. # and ### vs. the control group; , , and vs. the HCT group or H2O2 group.
(a) |
(b) |
(c) |
(d) |
(e) |