Downregulation of the Proton-Activated Cl- Channel TMEM206 Inhibits Malignant Properties of Human Osteosarcoma Cells

Figure 2

TMEM206 downregulation inhibit OS cell proliferation. (a) The expression of TMEM206 in U2OS cell line were detected by fluorescence staining. Scale bar: 20 μm. (b) The expression of TMEM206 in U2OS and MG63 cells were detected by western blot analysis (, as comparing with SC-shRNA group, respectively). (c) EdU staining assays were performed to determine the growth of TMEM206-shRNA-transfected U2OS and MG63 cells (, , as comparing with SC-shRNA group, respectively). (d) CCK-8 assay showed that downregulation of TMEM206 influence the proliferation rates in U2OS and MG63 cells measured at 1, 2, 3, and 4 day after transfection (, as comparing with SC-shRNA group, respectively). (e) Colony formation assay showed that knockdown TMEM206 inhibited cell colony formation (, as comparing with SC-shRNA group, respectively). GAPDH was used as an internal control. The data are represented as from 3 replicate experiments.

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