Downregulation of the Proton-Activated Cl- Channel TMEM206 Inhibits Malignant Properties of Human Osteosarcoma Cells

Figure 4

Upregulation of TMEM206 promotes the expression of β-catenin. (A) IHC results of the expression of β-catenin in osteosarcoma tissues and paired para-tumor tissues. Scale bar: 50 μm. (b) WB results indicated β-catenin expression was significantly elevated in patients with advanced stage (, , , as comparing with para-tumor tissue group, respectively). (c) U2OS and MG63 cells were conducted with a firefly luciferase reporter assay, and the firefly luciferase activity of each sample was normalized to the Renilla luciferase activity (, as comparing with pcDNA-Con group, respectively). (d) Upregulation of TMEM206 enhanced the β-catenin stain intensity in MG63 cells. Scale bar: 50 μm. (e) Upregulation of TMEM206 enhanced the expression level of β-catenin protein. GAPDH was used as an internal control. The data are represented as in (b) and represented as in (c).

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