Circular RNA circPHKA2 Relieves OGD-Induced Human Brain Microvascular Endothelial Cell Injuries through Competitively Binding miR-574-5p to Modulate SOD2

Figure 3

circPHKA2 inhibited apoptosis, ER stress, and oxidative stress of HBMEC after OGD. OGD-induced HBMEC were pretransfected with oe-circPHKA2 or NC, compared with control HBMEC transfected with NC. (a) FCM identified percentage of apoptotic cells. (b, c) Western blotting detected relative protein expression of Bcl-2, Bax, GRP78, CHOP, caspase-12, and β-actin. (d–f) Assay kits of ROS, MDA, and SOD examined relative ROS level, MDA level, and SOD activity, respectively. (g) Western blotting detected relative SOD2 protein expression, normalized to β-actin. .

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