Recombinant Human Growth Hormone Inhibits Lipotoxicity, Oxidative Stress, and Apoptosis in a Mouse Model of Diabetic Cardiomyopathy

Figure 2

Treatment with rhGH improved cardiac function and alleviated cardiac tissue damage in db/db mice. (a) rhGH improved cardiac function in db/db mice. Typical echocardiogram images are shown in the figure. Quantified of LV ejection fraction (EF), LV fraction shortening (FS), LV internal dimension diastole (LVIDd), LV internal dimension systole (LVIDs), LV anterior wall systole thickness (LVAWs), LV anterior wall diastole thickness (LVAWd), LV posterior wall systole thickness (LVPWs), and LV posterior wall diastole thickness (LVPWd) are shown in the bar graph. per group. vs. db/db group and vs. db/db group. (b, c) HE staining for tissue structure damage and Masson’s trichrome staining for collagen deposition in the cardiac tissue. (d) WGA staining (green fluorescence) and DAPI staining (blue fluorescence) for cardiomyocyte hypertrophy in the cardiac tissue. (e) Quantification of the relative fibrotic area and the cross areas of cardiomyocytes. Scale bar: 100 μm. The arrows indicate positively stained. per group. vs. db/db+rhGH group and ^# vs. db/db group.