Research Article

CaMK II Inhibition Attenuates ROS Dependent Necroptosis in Acinar Cells and Protects against Acute Pancreatitis in Mice

Figure 7

KN93 mitigated the severity of PDL-induced AP and prolonged survival of mice. (a) Representative HE staining of pancreas in SAP model induced by PDL in magnifications 100x and 400x. Scar μM. (b) Histological scores of pancreas in SAP model induced by PDL. (c) Protein levels of RIP3 and p-MLKL in pancreas were analyzed by Western blotting. (d) Serum levels of amylase and lipase in SAP model induced by PDL. (e) Relative protein expression GAPDH and MLKL were used as control for protein loading, respectively. (f) For survival studies, KN93 was applied to PDL mice 1 day to 4 days after surgery and survivorship curve of mice in SAP model induced by PDL. each group. , , and . PDL: pancreatic duct ligation. H represents high-dose KN93 (20 mg/kg).
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