Research Article
Zinc Regulates Glucose Metabolism of the Spinal Cord and Neurons and Promotes Functional Recovery after Spinal Cord Injury through the AMPK Signaling Pathway
Figure 1
Zinc inhibited apoptosis of PC12 neurons. (a–d) The MTT results of PC12 cells stimulated by H2O2 (). (e) Photos of PC12 cells treated with different concentrations of H2O2 for different time. (f) Using MTT to detect the cell viability of PC12 cells after zinc treatment (). (g–i) The expression levels of cleaved caspase 3, Bax, and Bcl-2 proteins were detected by WB. β-Actin was used as internal controls for WB (). (j, k) Apoptosis was detected using the MMP and Apoptosis Detection Kit (, ). (l, m) Flow cytometry results of apoptosis (). Data are represented as the . , , and .
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