Research Article

Protective Effect of Mitochondrial ND2 C5178A Gene Mutation on Cell and Mitochondrial Functions

Figure 4

Detection of mitochondrial OCR in mutant and control cell lines. (a) OCR curves of mutant and control cell lines after treatment with various inhibitors. The compounds (oligomycin, FCCP, and a mix of rotenone and antimycin A) are serially injected to measure ATP production, maximal respiration, and nonmitochondrial respiration, respectively. Proton leak and spare respiratory capacity are then calculated using these parameters and basal respiration. (b) Statistical analysis of the basic OCR, ATP-coupled OCR, maximum OCR, proton leak OCR, buffered OCR, and nonmitochondrial OCR of mutant and control cell lines ().
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